TYK2 Knockout cell line (THP-1)

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location: Home > Products > KO Cell Lines > TYK2 Knockout cell line (THP-1)
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TYK2 Knockout cell line (THP-1) Order

TYK2 Knockout cell line (THP-1)
Catalog#
YKO-HT1121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TYK2 Knockout cell line (THP-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TYK2 Knockout cell line (THP-1)
Species
Human
Gene Name
TYK2
Gene ID
7297
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 7 (based on transcript TYK2-204)
Validation: PCR+Sanger Sequencing
TYK2 Knockout cell line (THP-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TYK2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the THP-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TYK2 gene knockout TYK2 Knockout cell line (THP-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TYK2 Knockout cell line (THP-1)
TYK2 Knockout cell line (THP-1)
Catalog#
YKO-HT1121
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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    Instruction: EZ-cryo™ Cell Freezing Medium

KO Cell Lines

  • TYK2 Knockout cell line (Hep G2)

    TYK2 Knockout cell line (Hep G2)

    Organism: Human
    Gene Name: TYK2
    Gene ID: 7297
    Size: 1*10^6
    Catalog#: YKO-HT2373
  • TYK2 Knockout cell line (HeLa)

    TYK2 Knockout cell line (HeLa)

    Organism: Human
    Gene Name: TYK2
    Gene ID: 7297
    Size: 1*10^6
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  • TYK2 Knockout cell line (HCT 116)

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    Organism: Human
    Gene Name: TYK2
    Gene ID: 7297
    Size: 1*10^6
    Catalog#: YKO-XN95287

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